✦ LIBER ✦

A simple, rapid, and sensitive procedure for the assay of endoproteases using coomassie brilliant blue G-250

✍ Scribed by M. Saleemuddin; Hassan Ahmad; Athar Husain

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 350 KB
Volume: 105
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90446-7

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✦ Synopsis

A rapid calorimetric method for the assay of proteolytic enzymes based on the binding of Coomassie brilliant blue G-250 to unhydrolyzed protein substrate is described. Considerable assay time is saved since the method does not require the separation of the hydrolyzed products from the undegraded protein substrate. The procedure is applicable to crude as well as purified preparations of various proteolytic enzymes and compares well with the procedure of M. L. Anson (1938, J. Gen. Physiol. 22, 79-89).

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