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A Coomassie Brilliant Blue G-250-Based Colorimetric Assay for Measuring Activity of Calpain and Other Proteases

✍ Scribed by M. Burokerkilgore; K.K.W. Wang


Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
478 KB
Volume
208
Category
Article
ISSN
0003-2697

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A rapid calorimetric method for the assay of proteolytic enzymes based on the binding of Coomassie brilliant blue G-250 to unhydrolyzed protein substrate is described. Considerable assay time is saved since the method does not require the separation of the hydrolyzed products from the undegraded pro