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Protein Determination in Permeabilized Yeast Cells Using the Coomassie Brilliant Blue Dye Binding Assay

โœ Scribed by C.A.A. Cordeiro; A.P. Freire

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
247 KB
Volume
223
Category
Article
ISSN
0003-2697

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The direct measurement of protein in cell suspensions using the Coomassie brilliant blue dyebinding assay demonstrated markedly lower values compared to those obtained with the Lowry assay. It is shown that the addition of small amounts of Triton X-100 or NaOH to the cell suspensions prior to additi