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Measurement of protein in cell suspensions using the Commassie brilliant blue dye-binding assay

✍ Scribed by Geir O. Gogstad; May-Britt Krutnes

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 381 KB
Volume: 126
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90527-9

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✦ Synopsis

The direct measurement of protein in cell suspensions using the Coomassie brilliant blue dyebinding assay demonstrated markedly lower values compared to those obtained with the Lowry assay. It is shown that the addition of small amounts of Triton X-100 or NaOH to the cell suspensions prior to addition of the dye reagent corrected this discrepancy. Standards of soluble proteins may be used for the quantitation of protein in cell suspensions with the dyebinding assay provided that the same amounts of Triton X-100 or NaOH are added to both the standards and the samples.

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