๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Measurement of protein by dye-binding assay in the presence of metrizamide

โœ Scribed by Geir O. Gogstad


Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
311 KB
Volume
106
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Coomassie blue protein dye-binding assay
โœ Thomas Marshall; Katherine M. Williams ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 216 KB

The solubility of the protein-Coomassie brilliant blue (CBB) complex formed upon Bradford (Anal. Biochem. 72, 248-254, 1976) or Sedmak and Grossberg (Anal. Biochem. 79, 544-552, 1977) protein assay has been investigated by centrifugation or filtration of the assay mix within 10 min of adding dye rea

Measurement of protein in cell suspensio
โœ Geir O. Gogstad; May-Britt Krutnes ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 381 KB

The direct measurement of protein in cell suspensions using the Coomassie brilliant blue dyebinding assay demonstrated markedly lower values compared to those obtained with the Lowry assay. It is shown that the addition of small amounts of Triton X-100 or NaOH to the cell suspensions prior to additi

Mechanism of Dye Binding in the Protein
โœ A.A. Waheed; K.Sridhar Rao; P.D. Gupta ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 97 KB

Eosin B and eosin Y have been used to estimate micro- and submicrogram quantities of proteins respectively as shown in our previous reports. In the present study we describe the mechanism of eosin binding to proteins. At pH lower than 3.0 the absorbance of unbound dye is greatly reduced. After the d

Adaptation of the dye-binding protein as
โœ Margaret G. Redinbaugh; Wilbur H. Campbell ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 331 KB

The dye-binding protein assay has been adapted for use with microtiter plates and a plate reader. The total volume of the assay was reduced to 0.2 ml, with equal volumes of sample. and diluted dye reagent being used. Because of the small volume, the assay is conservative of both protein sample and r