The solubility of the protein-Coomassie brilliant blue (CBB) complex formed upon Bradford (Anal. Biochem. 72, 248-254, 1976) or Sedmak and Grossberg (Anal. Biochem. 79, 544-552, 1977) protein assay has been investigated by centrifugation or filtration of the assay mix within 10 min of adding dye rea
Measurement of protein by dye-binding assay in the presence of metrizamide
โ Scribed by Geir O. Gogstad
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 311 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
The direct measurement of protein in cell suspensions using the Coomassie brilliant blue dyebinding assay demonstrated markedly lower values compared to those obtained with the Lowry assay. It is shown that the addition of small amounts of Triton X-100 or NaOH to the cell suspensions prior to additi
Eosin B and eosin Y have been used to estimate micro- and submicrogram quantities of proteins respectively as shown in our previous reports. In the present study we describe the mechanism of eosin binding to proteins. At pH lower than 3.0 the absorbance of unbound dye is greatly reduced. After the d
The dye-binding protein assay has been adapted for use with microtiter plates and a plate reader. The total volume of the assay was reduced to 0.2 ml, with equal volumes of sample. and diluted dye reagent being used. Because of the small volume, the assay is conservative of both protein sample and r