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Modified Assay for the Measurement of Biotin in the Presence of Heparin

โœ Scribed by V.D. Nadkarni; R.J. Linhardt


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
297 KB
Volume
227
Category
Article
ISSN
0003-2697

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A competitive binding assay for biotin, biocytin, and desthiobiotin utilizing a genetically engineered enzyme-ligand conjugate is described herein. This assay is unique in that the enzyme-ligand conjugate consists of the streptavidin binding peptide Strep-tag II, which mimics the binding of biotin t