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A linear regression method for the study of the Coomassie brilliant blue protein assay

✍ Scribed by Yong-ju Wei; Ke-an Li; Shen-yang Tong

Book ID
117224037
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
550 KB
Volume
44
Category
Article
ISSN
0039-9140

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A sensitive assay procedure for the determination of microgram quantities of immobilized proteins is described. The procedure is based on the property of Coomassie blue G-250 to bind strongly yet reversibly to proteins. The assay involves incubation of the immobilized protein with a solution contain