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A method for the quantitative determination of protein incorporated in solubilizable polyacrylamide gels

✍ Scribed by Johan Tas; Alfons C.J. de Vries; RenéG. Berndsen

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
483 KB
Volume
100
Category
Article
ISSN
0003-2697

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✦ Synopsis

Dense and light polyacrylamide gels containing N,N'-( 1,2-dihydroxyethylene)bisacrylamide (DHEBA) or N,N'-diallyltartardiamide (DATD) as the crosslinker have been tested for their solubilization properties. Both types of gel can be dissolved in 10 mM periodic acid. The time and temperature required for complete dissolution of slabs of DHEBA crosslinked gels (12 h at WC), however, greatly exceed those required for dissolving slabs of DATDpolyacrylamide gel (0.5 h at 22°C). Bovine serum albumin kept under the respective dissolving conditions gave a lower response in the Lowry protein assay in instances where hot periodic acid had been used. Nearly independent of the type and concentration of their constituents, the different dissolved polyacrylamide gels interfere slightly with the Lowry assay by causing some "aspecific" color development. A method is outlined enabling a reliable quantitative determination of protein incorporated in DHEBA or DATD crosslinked polyacrylamide gels.

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