Dense and light polyacrylamide gels containing N,N'-( 1,2-dihydroxyethylene)bisacrylamide (DHEBA) or N,N'-diallyltartardiamide (DATD) as the crosslinker have been tested for their solubilization properties. Both types of gel can be dissolved in 10 mM periodic acid. The time and temperature required
A High-Sensitivity, Single-Gel, Polyacrylamide Gel Electrophoresis Method for the Quantitative Determination of Glutathione Reductases
β Scribed by Bin Ye; Carlos Gitler; Jonathan Gressel
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 341 KB
- Volume
- 246
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A Simple and Sensitive Method for Detection of L-Asparaginase by Polyacrylamide Gel Electrophoresis n-Asparaginase (n-asparagine amidohydrolase, EC 3.5.1.1) is an enzyme which has been applied as a potent antiproliferative drug in neoplastic diseases, particularly in leukemias. The enzyme was demons
A simple method for protein recovery from gel slices following polyacrylamide gel electrophoresis is proposed which requires neither additional devices nor much skill. The method is based on reversed electrophoresis using a discontinuous conductivity gradient which is in turn maintained by a gradien
A method is described for determination of carbohydrate and protein contents of glycoproteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then electroblotted onto polyvinylidene difluoride (PVDF) membranes. Blots were stained, and appropriate pieces of PVDF membranes w
A chromogenic substrate autography is described which allows characterization and quantification of serine proteases in crude systems after sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectric focusing. Separation of samples containing proteases by either method is followed by an