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A simple and rapid method for the quantitative isolation of proteins from polyacrylamide gels

✍ Scribed by M. Otto; Maja Šnejdárková

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
291 KB
Volume
111
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple method for protein recovery from gel slices following polyacrylamide gel electrophoresis is proposed which requires neither additional devices nor much skill. The method is based on reversed electrophoresis using a discontinuous conductivity gradient which is in turn maintained by a gradient in density. Protein recovery is possible within a small volume and approaches the theoretical yield. A large number of slices can be processed simultaneously.

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