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Method for determination of specific activity of proteins in polyacrylamide gels

✍ Scribed by David H. Alpers; Robert Glickman

Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
419 KB
Volume
35
Category
Article
ISSN
0003-2697

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✦ Synopsis

Disc electrophoresis has been widely used as .a procedure for separating proteins. In many instances the gels have been analyzed for radioactivity and for protein content. However, the only available method for determining protein content has been microdensitometry, which is complex and requires expensive equipment. The present technique is a simple and reproducible method which permits accurate measurement of protein bands containing as little as 10 pg protein.

MESTHODS Electrophoresis

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