Polyacrylamide gel interference of the lowry method for protein determination

✍ Scribed by P.A. Fields; L.H. Larkin

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 406 KB
• Volume: 88
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90411-6

✦ Synopsis

Certain reagents utilized in the formation of polyacrylamide gels are shown to interfere in the Lowry assay for protein. Acrylamide (3-30%) and potassium ferrocyanide (O.OOlS-0.0105%) produced a linear response in color formation. Both compounds are capable of reducing the phenol reagent in the absence of copper and the interference can be compensated for by employing the appropriate blank. An extract of polymerized and electrophoresed gels also interferes in the Lowry assay, however, this increased color formation cannot be corrected by using a gel extract blank. Under the conditions studied, filtration, centrifugation, and dialysis did not sufficiently remove the acrylamide fines responsible for the interference.