Universal Solid Supports for the Synthesis of Oligonucleotides via a Transesterification of H -phosphonate Diester Linkage
✍ Scribed by Ferreira, Fernando; Meyer, Albert; Vasseur, Jean-Jacques; Morvan, François
- Book ID
- 125961491
- Publisher
- American Chemical Society
- Year
- 2005
- Tongue
- English
- Weight
- 463 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0022-3263
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A novel solid phase synthesis of 3'-phosphorylated oligonuclcotides is described. The chain assembly is carried out by phosphoramidite strategy on solid support 2, which allows a mild and fast release of the oligonueleotide in solution. The applicability of the method is demonstrated by preparation
## Abstract Oligonucleotides containing terminal phosphate groups serve as useful intermediates for different applications in molecular and cell biology, as well as for diagnostic purposes. The chemical synthesis of these derivatives has been an important topic of oligonucleotide research. We repor