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A Novel Oxime-Derived Solid Support for the Synthesis of 3-Phosphorylated Oligonucleotides

✍ Scribed by Simon M. Langenegger; Luzia Moesch; François Natt; Jonathan Hall; Robert Häner

Publisher: John Wiley and Sons
Year: 2003
Tongue: German
Weight: 92 KB
Volume: 86
Category: Article
ISSN: 0018-019X
DOI: 10.1002/hlca.200390292

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✦ Synopsis

Abstract

Oligonucleotides containing terminal phosphate groups serve as useful intermediates for different applications in molecular and cell biology, as well as for diagnostic purposes. The chemical synthesis of these derivatives has been an important topic of oligonucleotide research. We report here a novel polystyrene‐based solid support bearing an α‐hydroxy oximate linker that allows the synthesis of 3′‐phosphorylated oligodeoxynucleotides as well as oligoribonucleotides in high quality. The formation of the phosphorylated oligonucleotides likely proceeds through an eliminative pathway with concurrent nitroso‐ene production.

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