𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Transformation of Streptococcus bovis protoplasts by plasmid DNA

✍ Scribed by M. Mareková; V. Kmet; P. Javorský


Book ID
111190203
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
271 KB
Volume
22
Category
Article
ISSN
0266-8254

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


High frequency transformation of Bacillu
✍ Chang, Shing ;Cohen, Stanley N. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra