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Transformation of Bacillus thuringiensis protoplasts by plasmid DNA

✍ Scribed by V.I. Miteva; N.I. Shivarova; R.T. Grigorova

Book ID
109311759
Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
229 KB
Volume
12
Category
Article
ISSN
0378-1097

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A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra

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✍ de Vos, Willem M. ;Venema, Gerard πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 603 KB

Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the