𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Improvements in the transformation of Bacillus subtilis protoplasts with plasmid DNA

✍ Scribed by C.P. Rubinstein; L.C. Moratinos; O.A. Coso; C. Sánchez-Rivas

Book ID
109314511
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
296 KB
Volume
56
Category
Article
ISSN
0378-1097

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Fate of plasmid DNA in transformation of
Fate of plasmid DNA in transformation of Bacillus subtilis protoplasts
✍ de Vos, Willem M. ;Venema, Gerard 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 603 KB

Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the

High frequency transformation of Bacillu
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA
✍ Chang, Shing ;Cohen, Stanley N. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra