✦ LIBER ✦

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA

✍ Scribed by Chang, Shing ;Cohen, Stanley N.

Publisher: Springer
Year: 1979
Tongue: English
Weight: 449 KB
Volume: 168
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00267940

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✦ Synopsis

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) transformants per microgram of supercoiled DNA. Plasmids constructed by in vitro ligation or endonuclease-generated fragments of linear plasmid DNA can also transform PEG-treated protoplasts, but at a lower frequency.

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