A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra
Polyethyleneglycol-induced transformation of Bacillus subtilis protoplasts by bacterial chromosomal DNA
✍ Scribed by Lévi-Meyrueis, Corinne ;Fodor, Katalin ;Schaeffer, Pierre
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 538 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0026-8925
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