A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra
Protoplast transformation of Staphylococcus carnosus by plasmid DNA
✍ Scribed by Götz, Friedrich ;Kreutz, Birgit ;Schleifer, Karl Heinz
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 325 KB
- Volume
- 189
- Category
- Article
- ISSN
- 0026-8925
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Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the
Two thermophilic strains of Lactobacillus were transformed by electroporation: ~.fermentum with a maximum of frequency of ixl05/ug of plasmid vector pPSC2nDNA and 1.4xl03/ug pSA3DNA. L.helveticus showed a very low ~requency of transformation, from 9 to 26 transformants/ug DNA in all the experiments