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Plasmid dna transformation ofLactobacillusstrains by electropermeabilization

✍ Scribed by Marisa Vescovo; F. Bosi; O. Borsani; V. Bottazzi

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
316 KB
Volume
5
Category
Article
ISSN
0951-208X

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✦ Synopsis

Two thermophilic strains of Lactobacillus were transformed by electroporation: ~.fermentum with a maximum of frequency of ixl05/ug of plasmid vector pPSC2nDNA and 1.4xl03/ug pSA3DNA. L.helveticus showed a very low ~requency of transformation, from 9 to 26 transformants/ug DNA in all the experiments carried out with both the vectors. While L.fermentum transformants were very stable, in L.helveticus the acquired plasmid was lost after 30-50 generations.

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