Transformation ofClostridiumthermohydrosulfuricumDSM 568 with plasmid DNA
โ Scribed by Erwin Soutschek-Bauer; Lydia Hartl; Walter L. Staudenbauer
- Publisher
- Springer Netherlands
- Year
- 1985
- Tongue
- English
- Weight
- 325 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0141-5492
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The possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium (Enterococcus faecalis) and two gram-negative bacteria (Escherichia coli and Pseudomonas putida) with plasmid DNA was investigated. E. faecalis protoplasts could be transformed by electroporati
Two thermophilic strains of Lactobacillus were transformed by electroporation: ~.fermentum with a maximum of frequency of ixl05/ug of plasmid vector pPSC2nDNA and 1.4xl03/ug pSA3DNA. L.helveticus showed a very low ~requency of transformation, from 9 to 26 transformants/ug DNA in all the experiments