The possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium (Enterococcus faecalis) and two gram-negative bacteria (Escherichia coli and Pseudomonas putida) with plasmid DNA was investigated. E. faecalis protoplasts could be transformed by electroporati
Transformation ofHaemophilus influenzaefollowing electroporation with plasmid and chromosomal DNA
โ Scribed by Jane K. Setlow; William L. Albritton
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 369 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0343-8651
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Using electroporation we have transformed Cellulomonas flavi~ena with a shuttle vector (pJA85) derived from the E. coli plasmid pUC8 and the Brevibacterium lactofermentum plasmid p0-LRS-~. Upon transformation this plasmid was found to be stable, not to undergo detectable deletion, and to express ant
The release of chromosomal and plasmid DNA from Acinetobacter calcoaceticus and Bacillus subtilis cultivated in minimal medium and broth over a period of 50 h was monitored and related to growth phase, autolysis, DNase production and natural competence. The released DNAs were biologically active in