✦ LIBER ✦
Transformation of the coryneform bacterium cellulomonas flavigena with plasmid DNA via electroporation
✍ Scribed by S. J. Alemohammad; J. Tony Pembroke
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 144 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
✦ Synopsis
Using electroporation we have transformed Cellulomonas flavi~ena with a shuttle vector (pJA85) derived from the E. coli plasmid pUC8 and the Brevibacterium lactofermentum plasmid p0-LRS-~. Upon transformation this plasmid was found to be stable, not to undergo detectable deletion, and to express antibiotic resistance markers originating in Brevibacterium.