๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Characterization of plasmid transformation in Bacillus subtilis: Kinetic properties and the effect of DNA conformation

โœ Scribed by Contente, Sara ;Dubnau, David

Publisher
Springer
Year
1979
Tongue
English
Weight
826 KB
Volume
167
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

โœฆ Synopsis

Transformation of competent cells of Bacillus subtilis with antibiotic resistance plasmid DNA has shown that (a) competence for plasmid and chromosomal DNA develops with similar kinetics; (b) DNA linearized with a variety of restriction endonucleases does not transform; (c) CCC plasmid DNA is inactivated for transformation by a single nick; (d) T4 ligase restores transforming activity to both nicked and linearized DNA; (E) CCC relaxed DNA is fully active in transformation; (f) the DNA concentration-dependence of plasmid transformation is first order; and (g) plasmid transformation proceeds with a low efficiency, requiring the uptake of 10(3) to 10(4) DNA molecules per transformant. Based on this information, a model for the processing of chromosomal, plasmid and transfecting DNA is proposed.

๐Ÿ“œ SIMILAR VOLUMES

Plasmid transformation in Bacillus subti
Plasmid transformation in Bacillus subtilis: Factors affecting the synapsis of donor and recipient DNA
โœ Ceglowski, Piotr ;Trautner, Thomas A. ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 259 KB

Hybrid plasmids were constructed in which the transcription of regions of inserted DNA was defined. Cells containing these plasmids were transformed with monomeric forms of a different hybrid plasmid, which contained, however, the same inserted DNA as the resident plasmid. The transformation frequen

Different specific activities of the mon
Different specific activities of the monomeric and oligomeric forms of plasmid DNA in transformation of B. subtilis and E. coli
โœ Mottes, Monica ;Grandi, Guido ;Sgaramella, Vittorio ;Canosi, Umberto ;Morelli, G ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 676 KB

(1) The low residual transforming activity in preparations of monomeric, supercoiled, circular (CCC) forms of the plasmids pC194 and pHV14 could be attributed to the presence in such isolates of a small number of contaminating multimeric molecules. (2) E. coli derived preparations of pHV14, as in vi