The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg
β Scribed by Haima, Peter ;Bron, Sierd ;Venema, Gerard
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 847 KB
- Volume
- 209
- Category
- Article
- ISSN
- 0026-8925
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Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h
Transformation of competent cells of Bacillus subtilis with antibiotic resistance plasmid DNA has shown that (a) competence for plasmid and chromosomal DNA develops with similar kinetics; (b) DNA linearized with a variety of restriction endonucleases does not transform; (c) CCC plasmid DNA is inacti