The previously cloned and sequenced streptokinase gene (skc) from Streptococcus equisimilis H46A was inserted into plasmid vectors capable of replication in Bacillus subtilis. The skc gene was expressed by use of its own transcription and translation signals which appeared to meet the stringent requ
High level of expression and stability of the cloned alkaline protease (aprA) gene in Bacillus subtilis
β Scribed by Taha I. Zaghloul; A. Abdelaziz; M.H. Mostafa
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 373 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0141-0229
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The recA gene of Proteus mirubilk (recApm) has been cloned into the Pat1 site of the Bacillus promoter-probe plasmid pPL603. When present on this plasmid, the rectlpm') gene is expressed in B. subtilie under the control of its own transcriptional and translational signals. It is concluded that the h
## Abstract Five independent DNA microarray experiments were used to study the gene expression profile of a 5βday __Bacillus subtilis__ airβliquid interface biofilm relative to planktonic cells. Both wildβtype __B. subtilis__ and its sporulation mutant (__ΞspoIIGB__::__erm__) were investigated to d
The constitutive MLS resistance plasmid pE3692 (2.4 kb) from S. uureus and pE3692-9 plasmid derived from pE3692 by in vitro deletion of the small HindIII-HindIII fragment Stat (0.3 kb) were introduced into S. uureus RN450 and B. subtilis UOT0277 strains. The pE3692 plasmid was completly stable in S.
## Abstract A novel metabolic engineering technique involving the redirection ofcellular carbon fluxes was employed to reduce acetate production in an __Escherichia coli__ culture. Metabolic engineering was achieved by cloning __E. coli__ the gene for the __Bacillus subtilis__ acetolactate synthase