TRANSFORMATION OF BACILLUS MEGATERIUM PROTOPLASTS BY PLASMID DNA
✍ Scribed by I. P. VOROBJEVA; I.A. KHMEL; I. ALFÖLDI
- Book ID
- 109300630
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 224 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra
Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the