THIAMIN AND RIBOFLAVIN ANALYSIS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
β Scribed by J. F. KAMMAN; T. P. LABUZA; J. J. WARTHESEN
- Book ID
- 108804552
- Publisher
- Institute of Food Technologists
- Year
- 1980
- Tongue
- English
- Weight
- 348 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0022-1147
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Phosphoric acid esters of riboflavin can be easily separated by reverse-phase high-performance liquid chromatography using eluants of 0.1 M ammonium formate in aqueous methanol. Commercial FMN preparations contained seven different flavin phosphates; the content of riboflavin S-phosphate was 70-7590
A procedure for the rapid, sensitive, and direct measurement of thiamin pyrophosphate (TPP) in erythrocytes using high-performance liquid chromatography (HPLC) is described. Human erythrocyte TPP levels from 17 normal healthy adults had a mean value of 123 + 27 @ml packed erythrocytes, ranging from