The measurement of erythrocyte thiamin pyrophosphate by high-performance liquid chromatography

✍ Scribed by L.G. Warnock

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 297 KB
• Volume: 126
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90533-4

✦ Synopsis

A procedure for the rapid, sensitive, and direct measurement of thiamin pyrophosphate (TPP) in erythrocytes using high-performance liquid chromatography (HPLC) is described. Human erythrocyte TPP levels from 17 normal healthy adults had a mean value of 123 + 27 @ml packed erythrocytes, ranging from 80 to 166 rig/ml. These values are in general agreement with TPP levels of control subjects using the enzymatic method developed previously by this laboratory. Statistical evaluation of the TPP levels using both of these methods on I3 samples of calf blood resulted in no significant difference (mean values of 114 f 22 and I I5 + 20). The TPP levels determined by the HPLC method on erythrocytes from rats fed a thiamindeficient diet fell rapidly during the first 9 days that the animals were on the diet. They had fallen out of the normal range by the 5th day. It remains to be determined whether this method can be applied to humans as an index of thiamin status.