A method has been developed for assay of aldehyde dehydrogenase (ALDH) in brain tissue or in other tissues containing low ALDH-activity. The aldehyde of dopamine was used as the substrate, and the 3,4-dihydroxyphenylacetic acid formed was measured using high-performance liquid chromatography (HPLC)
Determination of Erythrocyte Aldehyde Dehydrogenase Activity by High-Performance Liquid Chromatography
β Scribed by T. Yamamoto; Y. Moriwaki; S. Takahashi; Y. Nasako; K. Hiroishi; K. Higashino
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 372 KB
- Volume
- 215
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A radiochemical reverse-phase-high-performance liquid chromatography-linked method to measure the activity of nicotinamide phosphoribosyltransferase (EC 2.4.2.12) in crude lysates of human red blood cells is described. The apparent Km for nicotinamide was in the micromolar range, much lower than tha
## Abstract This work suggests an HPLC method for qualitative and quantitative determination of __N__^Ξ΅^(2βaminoβ2βcarboxyethyl)βLβlysine (LAL). LAL was released from total hydrolysates of various proteins of animal origin and derivatized with dansyl chloride. The performance of two different colum
A highly sensitive and specific assay method for cystamine using high-performance liquid chromatography has been developed. The method is based on postcolumn derivatization of cystamine with o-phtalaldehyde in the presence of 2-mercaptoethanol and sodium hypochlorite. The separation of cystamine was