Determination of cystamine by high-performance liquid chromatography

✍ Scribed by Shuji Ida; Yoshiko Tanaka; Seitaro Ohkuma; Kinya Kuriyama

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 412 KB
• Volume: 136
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90229-x

✦ Synopsis

A highly sensitive and specific assay method for cystamine using high-performance liquid chromatography has been developed. The method is based on postcolumn derivatization of cystamine with o-phtalaldehyde in the presence of 2-mercaptoethanol and sodium hypochlorite. The separation of cystamine was achieved using a cation exchange column (ISC-OS/SO504). The assay was linear over the concentration range of 2 to 200 pmol. For the application of this assay method to biological materials, the pretreatment with a cation exchange column (Dowex 5OWX8) was necessary to remove interfering o-phthalaldehyde-reactive substances. Since cysteamine in biological materials was quantitatively converted to cystamine during these sampling procedures, this method was found to be suitable for assaying the cysteamine plus cystamine content in various organs and tissues. The cysteamine-cystamine content in various tissues of rat determined by the present assay method has been presented.