Fluorometric determination of haloperidol by high performance liquid chromatography

✍ Scribed by Tsuneyoshi, T.

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 226 KB
• Volume: 9
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240090412

✦ Synopsis

Chromatograms of 2% test mixture with semimicrobore system. (a) Low dispersion flow path (b) Dead volume 1/16 in.-1/16 in. coupling in Components: 1 rhamnose; 2 fructose; 3 glucose; 4 sucrose; 5 lactose. 0 10 m i n 0 mi " 1 0

4 Conclusion

Semi-microbore columns can be used with modified commercial refractive index detectors to give a level of performance comparable to that of analytical LC columns linked to conventional R1 detectors. For efficient operation at the small-bore scale, extra-column dispersion must be reduced to a minimum. However, provided that narrow-bore linking tubing is employed, semi-microbore system performance is not critically dependent on linking tube 1ength.Circulating watertemperature control is not essential when using 2 mm columns with the mobile phase and flow rate stated in this note.