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Fluorometric detection and determination of carbohydrates by high-performance liquid chromatography using ethanolamine

โœ Scribed by Takehiko Kato; Toshio Kinoshita

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
357 KB
Volume
106
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Reaction of a mixture of ethanolamine and boric acid with reducing sugars gave intense fluorescence. Application of this reaction to the postcolumn derivatization in high-performance liquid chromatography provided a rapid, sensitive, and simple detection and determination of carbohydrates under a mild reaction condition. Separation and determination of carbohydrates in enzymatic hydrolysate of amylose and human serum are also described.

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