✦ LIBER ✦

A fluorometric determination of N-methyl-4-phenylpyridinium ion, using high-performance liquid chromatography

✍ Scribed by Makoto Naoi; Tsutomu Takahashi; Toshiharu Nagatsu

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 406 KB
Volume: 162
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90431-3

No coin nor oath required. For personal study only.

✦ Synopsis

A simple and sensitive assay procedure for the quantitation of N-methyl-4-phenylpyridinium ion (MPP+), a neurotoxin, was devised using its fluorescence and high-performance liquid chromatography (HPLC). The thtorescence intensity of MPP+ was several thousand times more than that of N-methyl-1,2,3,6-tetrahydropyridine (MPTP), a metabolic precursor of MPP+. This method was found to be sensitive enough to measure less than 10 pmol MPPf without using HPLC and 10 fmol using HPLC. The oxidation of MPTP by monoamine oxidase in human brain synaptosomal mitochondria was examined by this assay method. This fluorometric-HPLC method should have broad application in the study of the neurotoxin MPP+. o 1987 Academic Press, Inc.

📜 SIMILAR VOLUMES

Fluorometric detection and determination

Fluorometric detection and determination of carbohydrates by high-performance liquid chromatography using ethanolamine

✍ Takehiko Kato; Toshio Kinoshita 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 357 KB

Reaction of a mixture of ethanolamine and boric acid with reducing sugars gave intense fluorescence. Application of this reaction to the postcolumn derivatization in high-performance liquid chromatography provided a rapid, sensitive, and simple detection and determination of carbohydrates under a mi

Fluorometric determination of adenine nu

Fluorometric determination of adenine nucleotides and adenosine by ion-paired, reverse-phase, high-performance liquid chromatography

✍ Alfonso Ramos-Salazar; Andrew D. Baines 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 389 KB

A sensitive and specific assay for measurement of adenine nucleotides and adenosine by paired-ion high-performance liquid chromatography is described. The I,N6-ethenoderivatives of ATP (c-ATP), ADP (+ADP), AMP (c-AMP). and adenosine (c-Ado), formed by reaction with chloroacetaldehyde at 37°C. were s

Fluorometric Determination of Phenol and

Fluorometric Determination of Phenol andp-Cresol in Urine by Precolumn High-Performance Liquid Chromatography Using 4-(N-Phthalimidinyl)benzenesulfonyl Chloride

✍ Yasuto Tsuruta; Shouji Watanabe; Hirofumi Inoue 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 92 KB

been developed. Because phenol and p-cresol are pres-A sensitive HPLC method for simultaneous determient in urine as sulfate or glucuronide conjugates, they nation of phenol and p-cresol in urine was developed. are hydrolyzed enzymatically or with acids, followed After acid hydrolysis of urine was c

Determination of dextromethorphan hydrob

Determination of dextromethorphan hydrobromide by high-performance liquid chromatography using ion-pair formation

✍ E. J. Kubiak; J. W. Munson 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 467 KB

A Hydrolysis Method Using Microwaves: De

A Hydrolysis Method Using Microwaves: Determination of N-Acetyl- and N-Glycolylneuraminic Acids in Biological Systems by Fluorometric High-Performance Liquid Chromatography

✍ A. Lagana; A. Marino; G. Fago; B.P. Martinez 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 433 KB

A simple, rapid, and extremely sensitive method for determining \(N\)-acetyl- and \(N\)-glycolylneuraminic acids in serum and in submandibular, sublingual, and parotid glands using high-performance liquid chromatography with a fluorometric detector is described. The neuraminic acids contained in the

Fluorometric determination of streptomyc

Fluorometric determination of streptomycin in serum by high-performance liquid chromatography using mobile phase containing fluorogenic reagent

✍ Hiroaki Kubo; Haozhi Li; Yoshie Kobayashi; Toshio Kinoshita 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 341 KB

A new postcolumn derivatization method for the fluorometric determination of streptomycin in serum by high-performance liquid chromatography is described. The serum was treated with 3.5% perchloric acid to precipitate proteins and the supernatant was directly injected into the chromatograph. Strepto