Fluorometric Determination of Phenol andp-Cresol in Urine by Precolumn High-Performance Liquid Chromatography Using 4-(N-Phthalimidinyl)benzenesulfonyl Chloride

✍ Scribed by Yasuto Tsuruta; Shouji Watanabe; Hirofumi Inoue

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 92 KB
• Volume: 243
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1996.0485

✦ Synopsis

been developed. Because phenol and p-cresol are pres-A sensitive HPLC method for simultaneous determient in urine as sulfate or glucuronide conjugates, they nation of phenol and p-cresol in urine was developed. are hydrolyzed enzymatically or with acids, followed After acid hydrolysis of urine was conducted, phenol by distillation or extraction with organic solvents prior and p-cresol were extracted with diisopropyl ether to measurement by GC or HPLC. In GC methods, pheand derivatized with 4-(N-phthalimidinyl)benzenesulnol and p-cresol are measured directly or after converfonyl chloride to give fluorescent sulfonyl esters. The sion to volatile derivatives, and in HPLC methods, they labeling reactions were completed at 75ЊC for 10 min. are directly measured without derivatization by means The fluorescent derivatives were separated on a reof normal phase column-uv detection ( 12) or reversedversed-phase column by a gradient elution with acetophase column-coulochemical detection (13), -fluoresnitrile-water and detected by fluorescence measurecence detection ( 14), or -uv detection (15-17). Howment of excitation at 300 nm and emission at 410 nm. ever, these GC and HPLC methods require relatively The detection limits (signal-to-noise ratio Å 3) for phelarge sample volumes (more than 1 ml) because of their nol and p-cresol were 0.17 and 0.25 pmol per injection, insufficient sensitivities for measuring small amounts