Fluorometric determination of streptomycin in serum by high-performance liquid chromatography using mobile phase containing fluorogenic reagent

✍ Scribed by Hiroaki Kubo; Haozhi Li; Yoshie Kobayashi; Toshio Kinoshita

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 341 KB
• Volume: 162
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90030-3

✦ Synopsis

A new postcolumn derivatization method for the fluorometric determination of streptomycin in serum by high-performance liquid chromatography is described. The serum was treated with 3.5% perchloric acid to precipitate proteins and the supernatant was directly injected into the chromatograph. Streptomycin was separated by reversed-phase, ion-pair chromatography with a mobile phase containing ninhydrin as a fluorogenic reagent, octanesulfonate, and 1,2ethanedisulfonate as counterions, and was detected by fluorescence using continuous-flow, postcolumn derivatization in an alkaline stream with ninhydrin in the mobile phase. This method is sensitive to 1 .O &ml using only 100 ~1 of serum. Comparison with a fluorescence polarization immunoassay gave a good correlation coefficient of 0.976. 0 1987 Academic Prw. Inc.