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Determination of catechol-O-methyltransferase activity in erythrocytes by high performance liquid chromatography with electrochemical detection

✍ Scribed by Eija Schultz; Erkki Nissinen; Seppo Kaakkola

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
383 KB
Volume
3
Category
Article
ISSN
0269-3879

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Purification of rat liver soluble catech
Purification of rat liver soluble catechol-O-methyltransferase by high performance liquid chromatography
✍ Tapio Korkolainen; Erkki Nissinen πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 324 KB πŸ‘ 1 views

The soluble form of catechol-0-methyltransferase (EC 2.1.1.6.) from rat liver was purified to homogeneity by high-performance anion-exchange chromatography and high-performance gel-filtration chromatography. The specific activity of the final pool was 270 U/mg protein. The purification was 1180-fold