✦ LIBER ✦

Thermolysin-catalyzed peptide synthesis in aqueous polymer two-phase systems

✍ Scribed by Hiromi Kitano; Yasushi Maeda; Masayo Yamamoto; Rie Izumida

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 458 KB
Volume: 197
Category: Article
ISSN: 1022-1352
DOI: 10.1002/macp.1996.021971215

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A proteolytic enzyme, thermolysin, was covalently immobilized to dextran which has a small number of carboxyl groups. Enzymatic synthesis of aspartame derivative (Boc‐Asp‐Phe‐OMe) using the dextran‐conjugated thermolysin was carried out in an aqueous polymer two‐phase system, where poly(ethylene glycol) (PEG) and dextran dissolved in water make a phase separation. The yield of the peptide catalyzed by the dextran‐conjugated thermolysin was higher than that using a native enzyme in the aqueous polymer two‐phase system partly due to the effect of the microenvironment around the modified enzyme and partly due to the “mass‐law” effect (the preferential dissolution of the peptide product into the PEG phase). The aqueous polymer two‐phase system would be very useful to carry out enzymatic organic syntheses in high yields.

📜 SIMILAR VOLUMES

Partition of ribosomes in two-polymer aq

Partition of ribosomes in two-polymer aqueous phase systems

✍ Sidney Pestka; David Weiss; Robert Vince 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 345 KB

Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia co/i ribosomes under equilibrium conditions.

ChemInform Abstract: The Effect of Organ

ChemInform Abstract: The Effect of Organic Solvents on Thermolysin-Catalyzed Peptide Synthesis of Z-Phe-Phe-O-Me in Aqueous Organic One-Phase Reaction System

✍ Koji Tadasa; M. Nurul Alam; Hiroshi Kayahara 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 26 KB 👁 2 views

Partitioning of host and recombinant cel

Partitioning of host and recombinant cells in aqueous two-phase polymer systems

✍ Concetta LaMarca; Abraham M. Lenhoff; Prasad Dhurjati 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 790 KB

Characterization of aqueous two-phase sy

Characterization of aqueous two-phase systems based on polydisperse phase forming polymers: Enzymatic hydrolysis of starch in a PEG–starch aqueous two-phase system

✍ Mats Larsson; Bo Mattiasson 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 411 KB 👁 1 views

A new method for determination of t h e binodial of an aqueous two-phase system, using spectrophotometric measurements of the turbidity, is described in this article. The method is especially designed to characterize phase systems composed of polydisperse phase components. It gives information about

Protease-Catalyzed peptide synthesis in

Protease-Catalyzed peptide synthesis in low-water organic two-phase systems and problems affecting it

✍ John M. Cassells; Peter J. Halling 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 568 KB

Study of cell-free protein synthesis in

Study of cell-free protein synthesis in aqueous two-phase systems

✍ Ewa Marszal; Martina Suchova; Premysl Konecny; William H. Scouten 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 709 KB

Protein synthesis in an aqueous two-phase system is reported here as a novel type of extractive bioconversion. Translation of BMV RNA was studied using either rabbit reticulocyte lysate or wheat-germ extract in aqueous dextran-PEG systems. Both polymers inhibited protein synthesis and the translatio