Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia co/i ribosomes under equilibrium conditions.
Partitioning of host and recombinant cells in aqueous two-phase polymer systems
β Scribed by Concetta LaMarca; Abraham M. Lenhoff; Prasad Dhurjati
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 790 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0006-3592
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π SIMILAR VOLUMES
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Aqueous two-phase systems containing two polymers are a mild separation method for biomolecules due to the high concentration of water (75-90% ) in the phases. The phase systems have been further developed by introduction of thermoseparating polymers which make recycling of phase components possible
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