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Partition of ribosomes in two-polymer aqueous phase systems

✍ Scribed by Sidney Pestka; David Weiss; Robert Vince

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
345 KB
Volume
71
Category
Article
ISSN
0003-2697

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✦ Synopsis

Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia co/i ribosomes under equilibrium conditions.

πŸ“œ SIMILAR VOLUMES

Fundamental studies of biomolecule parti
Fundamental studies of biomolecule partitioning in aqueous two-phase systems
✍ Alan D. Diamond; James T. Hsu πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 883 KB

Phase diagram data at 4Β°C was determined for the aqueous two-phase systems composed of polyethylene glycol, dextran, and water. The Flory-Huggins theory of polymer thermodynamics was used to correlate partitioning of biomolecules in these aqueous two-phase systems resulting in a simple linear relati