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Study of cell-free protein synthesis in aqueous two-phase systems

✍ Scribed by Ewa Marszal; Martina Suchova; Premysl Konecny; William H. Scouten

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 709 KB
Volume: 8
Category: Article
ISSN: 0952-3499
DOI: 10.1002/jmr.300080126

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✦ Synopsis

Protein synthesis in an aqueous two-phase system is reported here as a novel type of extractive bioconversion. Translation of BMV RNA was studied using either rabbit reticulocyte lysate or wheat-germ extract in aqueous dextran-PEG systems. Both polymers inhibited protein synthesis and the translation system partitioned into both phases. In the rabbit reticulocyte two-phase system, protein synthesis reached 30% of that in free solution, while in wheat-germ extract it was 60% of that in free solution. Protein was found mainly in the dextran (lower) phase but its partitioning was related to the polymer concentration, and molecular weight, as well as the ionic strength of the system. Protein synthesis was highly influenced by PEG concentration, potassium chloride concentrtation, and dextran molecular weight.

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