Protein adsorbents intended for use in aqueous two-phase systems
β Scribed by Per O. Hedman; Jan-Gunnar Gustafsson
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 420 KB
- Volume
- 138
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
Preparation of adsorbents with high partition coefficients in polyethylene glycol-dextran and polyethylene glycol-phosphate systems is described. These adsorbents may be used to carry to carry proteins away from the insoluble cell fragments generated during cell disruption. By chromatographic elution, proteins may be selectively desorbed in a reduced volume.
π SIMILAR VOLUMES
## Abstract **BACKGROUND:** Aqueous twoβphase extraction (ATPE) has many advantages as an efficient, inexpensive largeβscale liquidβliquid extraction technique for protein separation. However, the realization of ATPE as a protein separation technology at industrial scales is rather limited due to t
Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating