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Characterization of aqueous two-phase systems based on polydisperse phase forming polymers: Enzymatic hydrolysis of starch in a PEG–starch aqueous two-phase system

✍ Scribed by Mats Larsson; Bo Mattiasson

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
411 KB
Volume
31
Category
Article
ISSN
0006-3592

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✦ Synopsis

A new method for determination of t h e binodial of an aqueous two-phase system, using spectrophotometric measurements of the turbidity, is described in this article. The method is especially designed to characterize phase systems composed of polydisperse phase components. It gives information about the area representing the transition from homogeneous solution to a two-phase system. The two-phase systems studied were first a conventional Dextran T40-polyethylene glycol 20M (PEG 20M) system, then a less-well-defined phase system based on PEG 20M and partially hydrolyzed starch. The PEG POM-starch system could be changed with respect to t h e volume ratio between the phases with time using hydrolytic enzymes, and the possibility of using the glucose released from the starch polymer is pointed out. Then the system is transformed to an extractive bioconversion where the bottom phase polymer also served as the substrate.

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