✦ LIBER ✦

Synthesis and functionalities of poly(N-vinylalkylamide). VII. A novel aqueous two-phase systems based on poly(N-vinylacetamide) and dextran

✍ Scribed by Akio Kishida; Shirou Nakano; Yoshihiro Kikunaga; Mitsuru Akashi

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 123 KB
Volume: 67
Category: Article
ISSN: 0021-8995
DOI: 10.1002/(sici)1097-4628(19980110)67:2<255::aid-app7>3.0.co;2-t

No coin nor oath required. For personal study only.

✦ Synopsis

It was found that a poly( N-vinylacetamide) (polyNVA) -dextran system formed two phases. The top was polyNVA-rich and the bottom phase was dextran-rich, which is the same as the poly(ethylene glycol) (PEG) -dextran system, and the phase separation occurred at a lower concentration than the PEG-dextran system. The protein separation in the polyNVA-dextran system was studied using myoglobin. Myoglobin was partitioned more in the bottom (dextran-rich) phase in a polyNVA-dextran system. The partition coefficient of the polyNVA-dextran system was smaller that of the PEG-dextran system, which suggests that the polyNVA-dextran system is superior to the PEG-dextran system for myoglobin separation.

📜 SIMILAR VOLUMES

Synthesis and functionality of poly(N-vi

Synthesis and functionality of poly(N-vinylalkylamide). X. A novel aqueous two-phase system based on thermosensitive polymers and dextran

✍ Akio Kishida; Yoshihiro Kikunaga; Mitsuru Akashi 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 111 KB 👁 1 views

The use of thermosensitive polymers in an aqueous two-phase system was studied. Poly(N-isopropylacrylamide) (PNIPAAm) and poly(N-vinylisobutyramide) (PNVIBA) were used as thermosensitive polymers. Both polymers could form aqueous two-phase with dextran, respectively. The phase diagrams of each syste

Synthesis and functionalities of poly(N-

Synthesis and functionalities of poly(N-vinylalkylamide). VI. A novel thermosensitive hydrogel crosslinked poly(N-vinylisobutyramide)

✍ Kazuo Suwa; Yoshihiro Wada; Akio Kishida; Mitsuru Akashi 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 168 KB 👁 2 views

A novel thermosensitive poly( N-vinylisobutyramide)(polyNVIBA) hydrogel was prepared by the copolymerization of N-vinylisobutyramide (NVIBA) with butylene-bis-NVA(B-BNVA) as a crosslinker in a high yield. The swelling transition behavior was examined in comparison with poly(N-isopropylacrylamide)(po

Design and synthesis of a two compartmen

Design and synthesis of a two compartment micellar system based on the self-association behavior of poly(N-acylethyleneimine) end-capped with a fluorocarbon and a hydrocarbon chain

✍ R. Weberskirch; J. Preuschen; H. W. Spiess; O. Nuyken 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 193 KB

A novel approach to biotransformations i

A novel approach to biotransformations in aqueous-organic two-phase systems: Enzymatic synthesis of alkyl β-[D]-glucosides using microencapsulated β-glucosidase

✍ Quan Yi; Douglas B. Sarney; Jeffrey A. Khan; Evgeny N. Vulfson 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 126 KB 👁 1 views

A novel approach to enzymatic biotransformations in aqueous-organic two-phase systems was developed where the aqueous phase was contained within permeable polymeric capsules suspended in organic solvent. Microencapsulated ␤-glucosidase, used as a model enzyme, was shown to retain its catalytic activ