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Synthesis and functionality of poly(N-vinylalkylamide). X. A novel aqueous two-phase system based on thermosensitive polymers and dextran

✍ Scribed by Akio Kishida; Yoshihiro Kikunaga; Mitsuru Akashi

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 111 KB
Volume: 73
Category: Article
ISSN: 0021-8995
DOI: 10.1002/(sici)1097-4628(19990923)73:13<2545::aid-app1>3.0.co;2-1

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✦ Synopsis

The use of thermosensitive polymers in an aqueous two-phase system was studied. Poly(N-isopropylacrylamide) (PNIPAAm) and poly(N-vinylisobutyramide) (PNVIBA) were used as thermosensitive polymers. Both polymers could form aqueous two-phase with dextran, respectively. The phase diagrams of each system were successfully obtained. Using myoglobin as a model protein, a preliminary separation study was performed. The separation ability of both polymers was higher than that from the poly(ethylene glycol)-dextran system. Protein separation ability appeared to be related to the hydrophilic/hydrophobic balance of the polymers. Both PNVIBA and PNIPAAm rich phases maintained their thermosensitivity after two-phase formation. PNVIBA and PNIPAAm are useful as polymers for a functional aqueous two-phase system.

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Synthesis and functionalities of poly(N-vinylalkylamide). VII. A novel aqueous two-phase systems based on poly(N-vinylacetamide) and dextran

✍ Akio Kishida; Shirou Nakano; Yoshihiro Kikunaga; Mitsuru Akashi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 123 KB 👁 2 views

It was found that a poly( N-vinylacetamide) (polyNVA) -dextran system formed two phases. The top was polyNVA-rich and the bottom phase was dextran-rich, which is the same as the poly(ethylene glycol) (PEG) -dextran system, and the phase separation occurred at a lower concentration than the PEG-dextr