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The use of an amino acid analyzer for the rapid identification and quantitative determination of chitosan ollgosaccharides

✍ Scribed by Jin-Jyi Chang; John H. Hash

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 349 KB
Volume: 95
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90773-5

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✦ Synopsis

The oligosaccharides of chitosan, glucosamine through chitohexaose, were separated and quantitated with the use of an amino acid analyzer. The oligosaccharides can be satisfactorily resolved with a step gradient using two buffers, but resolution is improved with a linear buffer gradient. Using experimentally determined color factors, the method permits the rapid quantitation and identification of small amounts of chitosan oligosaccharides.

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