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An improved method for the determination of galactosaminitol, glucosaminitol, glucosamine, and galactosamine on an amino acid analyzer

✍ Scribed by Pi-wan Cheng; Thomas F. Boat

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 381 KB
Volume: 85
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90300-7

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✦ Synopsis

Existing methods for the separation and quantitation of galactosaminitol, glucosaminitol, glucosamine, and galactosamine with an amino acid analyzer have been improved substantially by (1) addition of 15% (v/v) 1-propanol to the citrate-borate buffer, (2) utilization of a two-buffer system, (3) doubling the length of the ninhydrin reaction coil, and (4) inclusion of an internal standard, r-amino-n-caproic acid, in each sample. These modifications decrease the time for the separation of these compounds to 110 min and increase the sensitivity for hexosaminitols to 3-4 nmol, and that for hexosamines, to 2 nmol.

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