A modification of determination for glucosamine and galactosamine in glycoprotein with the amino acid analyzer: Application to total acid hydrolyzate of rat renal glomerular basement membrane
✍ Scribed by Kimie Murayama; Noriko Shindo; Hikaru Koide
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 214 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A procedure was described for the rapid determination of glucosamine and galactosamine in total acid hydrolyzate of rat renal glomerular basement membrane (GBM) by the use of amino acid analyzer. Glucosamine and galactosamine, as well as other amino acids in glycoprotein hydrolyzate, were well identified and estimated simultaneously by using a short column of HITACHI I-PF-B spherical resin, eluted with a pH 6.09 buffer containing 8% methanol at 38°C.
Total time consumed for elution of galactosamine was 60 rain. This method is ideal for the separation of small amount of galactosamine from hydroxylysine-rich materials.