An improved procedure for high-sensitivity microsequencing: Use of aminoethyl aminopropyl glass beads in the beckman sequencer and the Ultrasphere ODS column for PTH amino acid identification
✍ Scribed by Ajit S. Bhown; John E. Mole; J.Claude Bennett
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 399 KB
- Volume
- 110
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
An improved procedure for high-sensitivity microsequencing is described. The method employs (i) aminoethyl aminopropyl glass beads for in situ purification of quadrol by absorbing a-amino group reactive impurities commonly found even in highly purified quadrol and (ii) an Altex S-pm Ultrasphere ODS column and highly purified methanol which is available commercially to identify phenylthiohydantoin derivatives of amino acids by highpressure liquid chromatography. The efficiency of the method has been demonstrated by the results of sequence analyses of peptides obtained by acid and cyanogen bromide digestions of viral proteins. A signiicant increase in the initial and repetitive yields has been consistently observed by this procedure.